The authors used RNA-seq (Illumina platform, 50-base single-end reads) to analyze the transcriptomes of dissected brains of males and females (4-6 days old). Flies from two populations (The Netherlands and Zimbabwe) were used. There were two biological replicates of each population and sex.
The text is freely available at:
Raw RNA-seq data are available under the GEO series GSE40907:
For Sebida, we performed an updated statistical analysis of the Catalan et al. short-read data, using NextGenMap and DESeq2, as described in:
Variation in the X:autosome distribution of male-biased genes among Drosophila melanogaster tissues and Its relationship with dosage compensation,,
(Genome Biol. Evol. 7: 1960-1971, 2015).
The M/F ratios are for the combined analysis of both populations. The FDR values represent the Benjami-Hochberg corrected P-values from DESeq2.