The authors used RNA-seq (Illumina platform) to analyze the D. melanogaster transcriptome across 27 different developmental stages. For Sebida, we include only data from 5-day-old males and females. More information is available from the modENCODE website
Expression data are presented in terms of fragments per kilobase of transcript per million fragments mapped (FPKM). In Sebida, the M/F ratio is the ratio of FPKM in males to that in females. In cases where FPKM was zero, a value of 1 was used to calculate the M/F ratio. For example, if male FPKM = 1000 and female FPKM = 0, the M/F ratio is given as 1000. This prevents undefined values or values of zero. The original FPKM values are also provided in the Sebida database.