M.J.Wyman, A.F.Agrawal, L.Rowe, Condition-dependence of the sexually dimorphic transcriptome in Drosophila melanogaster, (Evolution 64: 1836-1848; 2010).

The authors used oligonucleotide arrays from the Canadian Drosophila Microarray Centre (CDMC_Drosophila_Oligo14k1/Oligo14k2) to investigate condition-dependent sexually dimorphic gene expression in adult D. melanogaster. They found that sexual dimorphism in expression was greater in high-condition populations (that is, those grown under optimal lab conditions). For Sebida, we included only data from the high-condition lines. For each sex, 2 genotypes were used and the microarray hybridizations were performed in 6 replicates. This resulted in a total of 12 hybridizations for each sex.

The raw microarray data are available from the GEO database under series GSE19973.

Although the authors performed their analysis using single-channel data in the original paper, for Sebida we paired the male and female data within genotype and replicate so that we could calculate male/female expression ratios to use for input into BAGEL.