J.F.Ayroles, M.A.Carbone, E.A.Stone, K.W.Jordan, R.F.Lyman, M.M.Magwire, S.M.Rollmann, L.H.Duncan, F.Lawrence, R.R.H.Anholt, T.F.C.Mackay, Systems genetics of complex traits in Drosophila melanogaster, (Nature Genet. 41: 299-307; 2009).

These authors used commercially available Affymetrix Drosophila 2.0 oligonucleotide arrays to assay gene expression in young adult (3-5 day) males and females of 40 inbred lines of Drosophila melanogaster. All of the lines were derived from a wild population in Raleigh, North Carolina, USA. Two biological replicates were performed for each line/sex. Expression was measured as the median log2 signal intensity of all "perfect match" probes (up to 14 per transcript). The data are available from the ArrayExpress database under the accession number E-MEXP-1594.

Differences in expression between males and females were tested by Analysis of Variance (ANOVA). The male/female expression ratio and the False Discovery Rate (FDR) for the sex effect for each gene is given in the Sebida entry. Because these experiments had very high replication, almost all genes show a significant difference in expression between the sexes, even if the fold-change between males and females is small. Note that the M/F ratio is given as a straight ratio and is not ln or log2 transformed. All data were taken from the supplementary material published online with the above article. For genes with multiple probe sets/transcripts, the value given in Sebida is the average over all probe sets per gene.